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png is a raritually lightweight image file created by the gimp, a free. free download – CutMaster Pro 2D 1.3.3.1. high quality cutmaster pro file -.Spectrophotometric detection of electrochemical-oxidative damage to DNA.
The potential significance of DNA oxidative damage in the aetiology of human disease is being appreciated. The development of a method to determine oxidative DNA damage to the nucleotide guanine has been investigated. The reaction of guanine with the peroxide potassium bromate in the presence of ethanol gives a stable formazan which is completely reversible. The degree of formazan formation was found to follow the (a) kinetics of guanine oxidation by the KMnO4-ferricyanide redox system and (b) guanine uptake by calf thymus DNA. Potassium bromate at a concentration of 100 mM completely oxidised 50 mumol DNA/L. Guanine oxidation to formazan was initially greater than thymidine oxidation but was completed by 150 min. Experiments have been carried out to determine the limits of oxidation of DNA by bromate at different pH and the reversibility of the oxidation. Significant increases in formazan formation were detected with 20 mumol potassium bromate/L DNA and 500 mumol hydrogen peroxide/L at pH 9.0. These levels of bromate also gave complete reversal of the damage when potassium bromate was removed by dialysis. The results indicate that in the presence of the oxidant system: (1) guanine is the limiting reagent with other DNA nucleotides having a much smaller oxidative potential, (2) oxidation of guanine to formazan is irreversible and is not simply a simple reduction of the guanine nucleotide by KMnO4, (3) potassium bromate causes complete oxidative damage of DNA.Q:

Is there any limit of how many questions one can post a day?

Is there any upper limit of how many questions a single user can post/ask a day?

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There is no limit at all. You can ask as many questions as you want per day.
The number of questions you can ask per day is based on a combination of several factors such as your available reputation and the number of days remaining in a particular week. The current maximum number of questions one can ask per day is 250, but
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